KARAKTERISASI BAKTERI DARI ISOLAT MINYAK GORENG SISA PAKAI DARI RUMAH MAKAN KELURAHAN TUNGGULWULUNG

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Karya Tulis Ilmiah

KARAKTERISASI BAKTERI DARI ISOLAT MINYAK GORENG SISA PAKAI DARI RUMAH MAKAN KELURAHAN TUNGGULWULUNG

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ABSTRAC

Karsad, Karina. 2020. Characterization of Bacteria from Used Cooking Oil Isolates from the Tunggulwulung Village Restaurant. Ilmah's Writing Works. D3 Study Program Medical Laboratory Technology, STIKes MAHARANI. Pembimbing: (1) Erni Yohani Mahtuti, S.Pd., M.Kes (2) Lilla Maria, Ns., M.Kep

Used cooking oil is cooking oil that has been used repeatedly and has changed color and smell. This is caused by several factors including absorption of odor by fat, the presence of hydrolysis and oxidation processes and due to microbial activity. The research objective was to obtain bacterial isolates that were able to degrade used cooking oil. This type of descriptive experimental research, with research methods include 1). Isolation of used cooking oil microbes, making pure culture and microscopic observation. 2). Characterization and biochemical tests were carried out by Gram stain examination. 3). Enzymatic test for amylase, protease, cellulolytic and triglycerides. The results proved that the identification of used cooking oil bacteria was found in the species Bacillus sp. The bacteria that can decompose the protease is the W3 sample, while in the amylase, cellulolytic and triglyceride test the bacteria are not able to degrade with the duration of 5 days of use after not being used. Suggestions for this research are sellers / the public not to use cooking oil repeatedly / more than 3 times and further research so that they can identify bacteria molecularly to find out the species of the genus Bacillus.

Keywords: Used cooking oil, Fat Degradation Bacteria


Detail Information

Item Type
Karya Tulis Ilmiyah
Penulis
KARINA KARSAD - Personal Name
Student ID
1614313453014
Dosen Pembimbing
Erni Yohani Mahtuti, S.Pd., M.Kes - - Dosen Pembimbing 1
Ns. Lilla Maria, S.Kep., M.Kep - - Dosen Pembimbing 2
Penguji
Kode Prodi PDDIKTI
Edisi
Published
Departement
Teknologi Laboratorium Medik
Kontributor
Bahasa
Indonesia
Penerbit StiKes Maharani Malang : Malang.,
Edisi
Published
Subyek
No Panggil
R 2020 TLM
Copyright
Institut Teknologi Nasional Bandung
Doi

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